English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/66291
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Fatty acid activation of the uncoupling proteins requires the presence of the central matrix loop from UCP1

AuthorsJiménez-Barbero, Jesús ; Ledesma, Amalia ; Zaragoza, Pilar ; González-Barroso, M. Mar ; Rial, Eduardo
KeywordsUncoupling protein
transporter
mutagenesis
mitochondria
fatty acid
Issue DateSep-2006
PublisherElsevier
CitationBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1757 (9-10) : 1292-1296 (2006)
AbstractNoradrenaline signals the initiation of brown fat thermogenesis and the fatty acids liberated by the hormone-stimulated lipolysis act as second messengers to activate the uncoupling protein UCP1. UCP1 is a mitochondrial transporter that catalyses the re-entry of protons to the mitochondrial matrix thus allowing a regulated discharge of the proton gradient. The high affinity of UCP1 for fatty acids is a distinct feature of this uncoupling protein. The uncoupling proteins belong to a protein superfamily formed by the mitochondrial metabolite carriers. Members of this family present a tripartite structure where a domain containing two transmembrane helices, linked by a long hydrophilic loop, is repeated three times. Using protein chimeras, where the repeats had been swapped between UCP1 and UCP3, it has been shown that the central third of UCP1 is necessary and sufficient for the response of the protein to fatty acids. We have extended those studies and in the present report we have generated protein chimeras where different regions of the second repeat of UCP1 have been sequentially replaced with their UCP2 counterparts. The resulting chimeras present a progressive degradation of the characteristic bioenergetic properties of UCP1. We demonstrate that the presence of the second matrix loop is necessary for the high affinity activation of UCP1 by fatty acids
Description5 páginas, 2 figuras, 1 tabla -- PAGS nros. 1292-1296
Publisher version (URL)http://dx.doi.org/10.1016/j.bbabio.2006.05.027
URIhttp://hdl.handle.net/10261/66291
DOI10.1016/j.bbabio.2006.05.027
ISSN0006-3002
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.