English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/65506
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Changes in cell/tissue organization and peroxidase activity as markers for early detection of graft incompatibility in peach/plum combinations
Autor : Zarrouk, Olfa ; Testillano, P.S. ; Risueño, María Carmen ; Moreno Sánchez, María Ángeles ; Gogorcena Aoiz, Yolanda
Palabras clave : Compatibility
cambium
union interface
isozyme profile
phenolic compounds
Fecha de publicación : ene-2010
Editor: American Society for Horticultural Science
Citación : Journal of the American Society for Horticultural Science 135(1):9-17(2010)
Resumen: Changes in cell and tissue organization and in peroxidase activity have been analyzed to find early markers to predict graft incompatibility occurrence in peach/plum combinations (Prunus persica/Prunus spp.) at 5 months after grafting in the dormancy period. Different compatible and incompatible peach/plum grafts were grown for 5 months in a nursery. The cellular study of the graft interface revealed structural changes associated with graft incompatibility symptoms. The main structural features were cambium cell disorganization, less differentiation of vascular tissues, degeneration of phloem and xylem cells, and accumulation of phenols at the graft interface after 5 months of graft development. The peroxidase study was performed during dormancy and the vegetative growth period, and revealed a significant increase in peroxidase activity in the incompatible unions, with significant differences between compatible and incompatible grafts. Analysis of gel profiles of nonbudded rootstocks and scions revealed an anodal isoperoxidase band [relative front (Rf) = 0.48] present in scions and compatible rootstocks, and another isoperoxidase band (Rf = 0.53) only present in the incompatible rootstocks. Our results show that the analysis of cell organization to detect early structural events and the evaluation of peroxidase activity at graft unions constituted feasible and convenient methods for early diagnosis of graft incompatibility. Also, it was suggested that the presence of band Rf = 0.48 in plum rootstocks and peach cultivars could be used as a marker to predict graft compatibility for peach scions and plum rootstocks
Descripción : 9 páginas, 3 figuras, 4 tablas -- PAGS nros. 9-17
URI : http://hdl.handle.net/10261/65506
ISSN: 0003-1062
Aparece en las colecciones: (CIB) Artículos
(EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
J. Am.Soc.Hortic Sci 135(1)(9-17).pdf1,59 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.