English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/65148
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Evaluation of a fast method based on the presence of two restriction sites in the mitochondrial ND5 (mt ND5) gene for the identification of scomber species

AuthorsMartínez, Iciar ; Velasco, Amaya ; Pérez Martín, Ricardo Isaac ; Foras, Eskil; Yamashita, Michiaki; González Sotelo, Carmen
KeywordsSpecies identification
Fish
Mackerel
Scomber
PCR
FINS
mt DNA
RFLP-PCR,
Saba
Caballa
Scombrus
Colias
Japonicus
Australasicus
Traceability
Issue Date2012
PublisherTaylor & Francis
CitationJournal of Aquatic Food Product Technology 21(4): 289-297 (2012)
AbstractThe purpose of this work was to evaluate the suitability of a method based on the presence of two restriction sites (for Hae III and Hindf I) in the mitochondrial NADH dehydrogenase subunit 5 (mt ND5) gene to identify Scomber species. The evaluation was performed on 144 reference and market samples by sequencing of the entire 505-bp fragment of the mt ND5 gene and of a 464-bp fragment of the Kocher fragment of the cytochrome b gene (mt Cytb). Sequence analysis of any of the two fragments allows the identification of each of the four Scomber species, but S. japonicus and S. colias had the same restriction sites at the ND5 amplicon and would not have been differentiated by this analysis. Similarly, loss of the Hae III site in some S. scombrus individuals would have misidentified them as not being Scomber. All the market products were correctly labeled except one acquired in Spain labeled as originating in the Atlantic and containing S. japonicus
Description9 páginas
Publisher version (URL)http://dx.doi.org/10.1080/10498850.2012.664804
URIhttp://hdl.handle.net/10261/65148
DOI10.1080/10498850.2012.664804
ISSN1049-8850
E-ISSN1547-0636
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Evaluation_fast_method.pdf173,16 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.