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Título: | Dissociation of single-stranded DNA from nucleosomes following modification with acetic anhydride |
Autor: | Jordano, Juan CSIC ORCID ; Montero, F.; Palacián, Enrique | Fecha de publicación: | 1984 | Editor: | Academic Press | Citación: | Biochemical and Biophysical Research Communications 121(3): 907-914 (1984) | Resumen: | Modification with acetic anhydride of nucleosomes from chicken erythrocytes at low ionic strength (less than 0.1 M NaCl) is accompanied by the formation of residual particles and the release of free DNA. This DNA has been identified as single-stranded by thermal denaturation, digestion with nuclease S1, and elution from hydroxyapatite. In contrast, if modification takes place at 0.6 M NaCl, the liberated DNA is mainly double-stranded. The release of the free energy stored in folded nucleosomal DNA, triggered by the weakening of lysine-DNA interactions which takes place upon modification, might be responsible for the observed denaturation of DNA at low ionic stregth. | URI: | http://hdl.handle.net/10261/64614 | Identificadores: | issn: 0006-291X e-issn: 1090-2104 |
Aparece en las colecciones: | (IRNAS) Artículos |
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