English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/63732
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Leaf patch clamp pressure probe measurements on olive leaves in a nearly turgorless state

AutorEhrenberger, W.; Rüger, S.; Rodríguez Domínguez, Celia M. ; Díaz-Espejo, Antonio ; Fernández Luque, José Enrique ; Moreno Onorato, F. J.; Zimmermann, D.; Sukhorukov, V. L.; Zimmermann, U.
Fecha de publicación2012
EditorThieme
CitaciónPlant Biology 14(4): 666-674 (2012)
ResumenThe non-invasive leaf patch clamp pressure (LPCP) probe measures the attenuated pressure of a leaf patch, P p, in response to an externally applied magnetic force. P p is inversely coupled with leaf turgor pressure, P c, i.e. at high P c values the P p values are small and at low P c values the P p values are high. This relationship between P c and P p could also be verified for 2-m tall olive trees under laboratory conditions using the cell turgor pressure probe. When the laboratory plants were subjected to severe water stress (P c dropped below ca. 50kPa), P p curves show reverse diurnal changes, i.e. during the light regime (high transpiration) a minimum P p value, and during darkness a peak P p value is recorded. This reversal of the P p curves was completely reversible. Upon watering, the original diurnal P p changes were re-established within 2-3days. Olive trees in the field showed a similar turnover of the shape of the P p curves upon drought, despite pronounced fluctuations in microclimate. The reversal of the P p curves is most likely due to accumulation of air in the leaves. This assumption was supported with cross-sections through leaves subjected to prolonged drought. In contrast to well-watered leaves, microscopic inspection of leaves exhibiting inverse diurnal P p curves revealed large air-filled areas in parenchyma tissue. Significantly larger amounts of air could also be extracted from water-stressed leaves than from well-watered leaves using the cell turgor pressure probe. Furthermore, theoretical analysis of the experimental P p curves shows that the propagation of pressure through the nearly turgorless leaf must be exclusively dictated by air. Equations are derived that provide valuable information about the water status of olive leaves close to zero P c.
URIhttp://hdl.handle.net/10261/63732
DOI10.1111/j.1438-8677.2011.00545.x
Identificadoresdoi: 10.1111/j.1438-8677.2011.00545.x
issn: 1435-8603
e-issn: 1438-8677
Aparece en las colecciones: (IRNAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.