Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/63648
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

The SRY-HMG box gene, SOX4, is a target of gene amplification at chromosome 6p in lung cancer

AutorBlanco, Sandra CSIC ORCID CVN; Sanz-García, Marta CSIC; Lazo, Pedro A. CSIC ORCID ; Sánchez-Céspedes, M.
Fecha de publicación2009
EditorOxford University Press
CitaciónHuman Molecular Genetics 18(7): 1343-1352 (2009)
ResumenThe search for oncogenes is becoming increasingly important in cancer genetics because they are suitable targets for therapeutic intervention. To identify novel oncogenes, activated by gene amplification, we analyzed cDNA microarrays by high-resolution comparative genome hybridization and compared DNA copy number and mRNA expression levels in lung cancer cell lines. We identified several amplicons (5p13, 6p22-21, 11q13, 17q21 and 19q13) that had a concomitant increase in gene expression. These regions were also found to be amplified in lung primary tumours. We mapped the boundaries and measured expression levels of genes within the chromosome 6p amplicon. The Sry-HMG box gene SOX4 (sex-determining region Y box 4), which encodes a transcription factor involved in embryonic cell differentiation, was overexpressed by a factor of 10 in cells with amplification relative to normal cells. SOX4 expression was also stronger in a fraction of lung primary tumours and lung cancer cell lines and was associated with the presence of gene amplification. We also found variants of SOX4 in lung primary tumours and cancer cell lines, including a somatic mutation that introduced a premature stop codon (S395X) at the serine-rich C-terminal domain. Although none of the variants increased the transactivation ability of SOX4, overexpression of the wildtype and of the non-truncated variants in NIH3T3 cells significantly increased the transforming ability of the weakly oncogenic RHOA-Q63L. In conclusion, our results show that, in lung cancer, SOX4 is overexpressed due to gene amplification and provide evidence of oncogenic properties of SOX4. © The Author 2009. Published by Oxford University Press. All rights reserved.
URIhttp://hdl.handle.net/10261/63648
DOI10.1093/hmg/ddp034
Identificadoresdoi: 10.1093/hmg/ddp034
issn: 0964-6906
e-issn: 1460-2083
Aparece en las colecciones: (IBMCC) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

98
checked on 22-mar-2024

WEB OF SCIENCETM
Citations

94
checked on 26-feb-2024

Page view(s)

327
checked on 28-mar-2024

Download(s)

99
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.