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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Fernández, Israel S. | - |
dc.contributor.author | Ständker, Ludger | - |
dc.contributor.author | Forssmann, Wolf Georg | - |
dc.contributor.author | Giménez-Gallego, Guillermo | - |
dc.contributor.author | Romero, Antonio | - |
dc.date.accessioned | 2012-12-20T09:05:11Z | - |
dc.date.available | 2012-12-20T09:05:11Z | - |
dc.date.issued | 2007-08 | - |
dc.identifier.citation | Acta Crystallographica, Section F 63(8):669-672(2007) | es_ES |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://hdl.handle.net/10261/63366 | - |
dc.description | 4 páginas, 6 figuras, 2 tablas -- PAGS nros. 669-672 | es_ES |
dc.description.abstract | Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20 000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 Å resolution and data were collected to 99.6% completeness at 8.9 Å. The crystal belongs to space group P212121, with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 Å. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells | es_ES |
dc.description.sponsorship | We thank Professor K. Murray for providing plasmid pR1-11E. X-ray data collection was supported by BM14UK/ESRF, Grenoble and SRS, Daresbury. WST was supported by a Die NorKen Stiftung Visiting Fellowship and KLH is the recipient of the Darwin Trust Scholarship | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | International Union of Crystallography | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | hepatitis B core particles | es_ES |
dc.subject | cryoprotectant | es_ES |
dc.subject | temperature jump | es_ES |
dc.subject | N-terminal fusion | es_ES |
dc.title | Crystallization and preliminary crystallographic studies of human kallikrein 7, a serine protease of the multigene kallikrein family | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1107/S1744309107031764 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1107/S1744309107031764 | es_ES |
dc.identifier.pmid | 17671364 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
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Acta Cristallographica F 63 (8) 669-672 (2007).pdf | 228,99 kB | Adobe PDF | Visualizar/Abrir |
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