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Title

Cloning of the sole (Solea senegalensis) growth hormone-encoding cDNA

AuthorsPendón, Carlos; Martínez-Barberá, Juan P.; Pérez-Sánchez, Jaume CSIC ORCID; Rodríguez Martínez, Ramón B.; Grenett, Hernán; Valdivia, Manuel M.
KeywordsRecombinant DNA
Fish
Solea senegalensis
Growth hormone
Evolution
Teleost
Northern blot
Pituitary gland
Issue DateAug-1994
PublisherElsevier
CitationGene 145(2): 237-240 (1994)
AbstractWe report here the complete nucleotide (nt) sequence of a cDNA clone encoding Solea senegalensis growth hormone (sGH) isolated from an expression library prepared from sole pituitary gland poly (A)+ RNA. The library was screened using a flounder GH cDNA. The cDNA sequence containing an insert of 769 nt was found to encode a polypeptide of 203 amino acids (aa), including a signal peptide of 17 aa. The 5′- and 3′-untranslated regions of the message are 17 and 119-nt long, respectively. Northern blot hybridization detected a 0.9-kb RNA species. The sGH cDNA sequence shows homologies of 80.9, 76.9, 73.8 and 64.2% with the GH of tuna, gilthead seabream, flounder and rainbow trout.
Description4 pages.
Publisher version (URL)http://dx.doi.org/10.1016/0378-1119(94)90012-4
URIhttp://hdl.handle.net/10261/6326
DOI10.1016/0378-1119(94)90012-4
ISSN0378-1119
Appears in Collections:(IATS) Artículos
(ICMAN) Artículos

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