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Title

Molecular characterization and localization of the RIC-3 protein, an effector of nicotinic acetylcholine receptor expression

AuthorsCastelán, Francisco; Castillo, Mar; Mulet Soler, José; Sala, Salvador; Sala, Francisco; Domínguez del Toro, Eduardo; Criado, Manuel
Issue Date2008
PublisherBlackwell Publishing
CitationJournal of Neurochemistry 105: 617-627 (2008)
AbstractThe RIC-3 protein acts as a regulator of acetylcholine nicotinic receptor (nAChR) expression. In Xenopus laevis oocytes the human RIC-3 (hRIC-3) protein enhances expression of α7 receptors and abolishes expression of α4β2 receptors. In vitro translation of hRIC-3 evidenced its membrane insertion but not the role as signal peptide of its first transmembrane domain (TMD). When the TMDs of hRIC-3 were substituted, its effects on nAChR expression were attenuated. A certain linker length between the TMDs was also needed for α7 expression enhancement but not for α4β2 inhibition. A combination of increased α7 receptor steady state levels, facilitated transport and reduced receptor internalization appears to be responsible for the increase in α7 membrane expression induced by hRIC-3. Antibodies against hRIC-3 showed its expression in SH-SY5Y and PC12 cells and its induction upon differentiation. Immunohistochemistry demonstrated the presence of RIC-3 in rat brain localized, in general, in places where α7 nAChRs were found. © 2007 The Authors.
URIhttp://hdl.handle.net/10261/63064
DOI10.1111/j.1471-4159.2007.05169.x
Identifiersdoi: 10.1111/j.1471-4159.2007.05169.x
issn: 0022-3042
e-issn: 1471-4159
Appears in Collections:(IN) Artículos
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