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Título: | Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment |
Autor: | McConnell, Michael J.; Pérez-Ordóñez, A.; Pérez-Romero, Pilar CSIC ORCID; Valencia Martín, Raquel CSIC; Lepe, José A. CSIC ORCID; Vázquez-Barba, I.; Pachón, Jerónimo CSIC ORCID | Fecha de publicación: | 2012 | Editor: | American Society for Microbiology | Citación: | Journal of Clinical Microbiology 50(4): 1412-1414 (2012) | Resumen: | A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria Copyright © 2012, American Society for Microbiology. All Rights Reserved. | URI: | http://hdl.handle.net/10261/63029 | DOI: | 10.1128/JCM.06566-11 | Identificadores: | doi: 10.1128/JCM.06566-11 issn: 0095-1137 |
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