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Title

Hypoosmotic- and pressure-induced membrane stretch activate TRPC5 channels

AuthorsGomis, Ana; Soriano, Sergio; Belmonte, Carlos; Viana, Félix
Issue Date2008
PublisherBlackwell Publishing
CitationJournal of Physiology 586(23): 5633-5649 (2008)
AbstractTransient receptor potential (TRP) channels mediate a wide array of sensory functions. We investigated the role of TRPC5, a poorly characterized channel widely expressed in the central and peripheral nervous system, as a potential osmosensory protein. Here we show that hypoosmotic stimulation activates TRPC5 channels resulting in a large calcium influx. The response to osmotically induced membrane stretch is blocked by GsMTx-4, an inhibitor of stretch activated ion channels. Direct hypoosmotic activation of TRPC5 is independent of phospholipase C function. However, the osmotic response is inhibited in a cell line in which PIP2 levels are reduced by regulated overexpression of a lipid phosphatase. The response was restored by increasing intracellular PIP2 levels through the patch pipette. The mechano-sensitivity of the channel was probed in the whole-cell configuration by application of steps of positive pressure through the patch pipette. Pressure-induced membrane stretch also activated TRPC5 channels, suggesting its role as a transducer of osmo-mechanical stimuli. We also demonstrated the expression of TRPC5 in sensory neurones which together with the osmo-mechanical characteristics of TRPC5 channels suggest its putative role in mechanosensory transduction events. © 2008 The Author. Journal compilation © 2008 The Physiological Society.
URIhttp://hdl.handle.net/10261/62970
DOI10.1113/jphysiol.2008.161257
Identifiersdoi: 10.1113/jphysiol.2008.161257
issn: 0022-3751
e-issn: 1469-7793
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