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Title

Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose

AuthorsÁlvaro-Benito, Miguel ; Abreu Felipe, Miguel A. de; Fernández Arrojo, Lucía ; Plou Gasca, Francisco José ; Jiménez-Barbero, Jesús ; Ballesteros, Antonio O.; Polaina, Julio; Fernández Lobato, María
KeywordsSchwanniomyces occidentalis
Invertase
β-Fructofuranosidase
6-Kestose
Fructosyltransferase
Prebiotic
oligosaccharides
Issue Date15-Oct-2007
PublisherElsevier
CitationJournal of Biotechnology 132(1): 75-81(2007)
Abstractβ-Fructofuranosidases are powerful tools in industrial biotechnology. We have characterized an extracellular β-fructofuranosidase from the yeast Schwanniomyces occidentalis. The enzyme shows broad substrate specificity, hydrolyzing sucrose, 1-kestose, nystose and raffinose, with different catalytic efficiencies (kcat/Km). Although the main reaction catalysed by this enzyme is sucrose hydrolysis, it also produces two fructooligosaccharides (FOS) by transfructosylation. A combination of 1H, 13C and 2D-NMR techniques shows that the major product is the prebiotic trisaccharide 6-kestose. The 6-kestose yield obtained with this β-fructofuranosidase is, to our concern, higher than those reported with other 6-kestose-producing enzymes, both at the kinetic maximum (76 g l−1) and at reaction equilibrium (44 g l−1). The total FOS production in the kinetic maximum was 101 g l−1, which corresponded to 16.4% (w/w) referred to the total carbohydrates in the reaction mixture
Description7 páginas, 3 figuras, 1 tabla -- PAGS nros. 75-81
Publisher version (URL)http://dx.doi.org/10.1016/j.jbiotec.2007.07.939
URIhttp://hdl.handle.net/10261/62747
DOI10.1016/j.jbiotec.2007.07.939
ISSN0168-1656
E-ISSN1873-4863
Appears in Collections:(CIB) Artículos
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