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Title

Defined DNA sequences promote the assembly of a bacterial protein into distinct amyloid nanostructures

AuthorsGiraldo, R.
KeywordsAmyloidogenesis
conformational changes
DNA binding
fiber assembly
RepA protein
Issue Date30-Oct-2007
PublisherNational Academy of Sciences (U.S.)
CitationProceedings of the National Academy of Sciences, 104 (44) : 17388-17393 (2007)
AbstractRepA, the replication initiator protein of Pseudomonas pPS10 plasmid, is made of two winged-helix (WH) domains. RepA dimers undergo a structural transformation upon binding to origin DNA sequences (iterons), resulting in monomerization and α-helix into β-strand conversion. This affects the N-terminal domain (WH1) and generates a metastable intermediate. Here it is shown that the interaction of short dsDNA oligonucleotides, including iteron or operator RepA targets, with the isolated WH1 domain promotes the assembly of different nanostructures. These range from irregular aggregates to amyloid spheroids and fibers. Their intrinsic order inversely correlates with the extent of the transformation induced by each DNA sequence on RepA. However, DNA is not a constituent of the assembled fibers, in agreement with the protein-only principle for amyloid structure. Thus, the RepA-WH1 domain on DNA binding mimics the behavior of the mammalian prion protein. The stretch of amino acids responsible for WH1 aggregation has been identified, leading to the design of mutants with enhanced or reduced amyloidogenicity and the synthesis of a peptide that assembles into a cross-β structure. RepA amyloid assemblies could have a role in the negative regulation of plasmid replication. This article underlines the potential of specific nucleic acid sequences in promoting protein amyloidogenesis at nearly physiological conditions
Description11 páginas, 5 figuras -- PAGS nros. 17383-17393
Publisher version (URL)http://dx.doi.org/10.1073/pnas.0702006104
URIhttp://hdl.handle.net/10261/62389
DOI10.1073/pnas.0702006104
ISSN1091-6490
Appears in Collections:(CIB) Artículos
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