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Title: Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous
Authors: Fernández Arrojo, Lucía, Marín Alberdi, Dolores, Gómez de Segura, María Aránzazu, Linde López, Dolores, Alcalde Galeote, Miguel, Gutiérrez Alonso, Patricia, Ghazi, Iraj, Plou Gasca, Francisco José, Fernández Lobato, María, Ballesteros, Antonio O.
Keywords: Prebiotics
Functional foods
Issue Date: 2007
Publisher: Elsevier
Abstract: The transglycosylation activity of a novel alpha-glucosidase from the basidiomycetous yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) was studied using maltose as glucosyl donor. The enzyme synthesized oligosaccharides with alpha-(1-2), alpha-(1-4) and alpha-(1-6) bonds. Using 200 g/l maltose, the yield of oligosaccharides was 53.8 g/l, with prebiotic oligosaccharides containing at least one alpha-(1-6) linkage (panose, 6-O-alpha-glucosyl-maltotriose and 6-O-alpha-isomaltosyl-maltose) being the major products (47.1 g/l). The transglycosylatying yield was 3.6 times higher than the observed with the alpha-glucosidase from Saccharomyces cerevisiae (53.8 vs. 14.7 g/l). Moreover, when increasing the maltose concentration up to 525 g/l, the maximum production of tri- and tetrasaccharides reached 167.1 g/l, without altering the percentage of oligosaccharides in the mixture. Compared with other microbial alpha-glucosidases in which the main transglycosylation product is a disaccharide, the enzyme from X. dendrorhous yields a final product enriched in trisaccharides and tetrasaccharides.
Publisher version (URL):
???metadata.dc.identifier.doi???: 10.1016/j.procbio.2007.08.007
Citation: Process Biochemistry Volume 42, Issue 11, November 2007, Pages 1530–1536
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(CBM) Patentes
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