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Título

Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous

AutorFernández Arrojo, Lucía; Marín Alberdi, Dolores; Gómez de Segura, María Aránzazu; Linde, Dolores CSIC ORCID ; Alcalde Galeote, Miguel CSIC ORCID ; Gutiérrez Alonso, Patricia; Ghazi, Iraj CSIC; Plou Gasca, Francisco José CSIC ORCID ; Fernández Lobato, María CSIC ORCID; Ballesteros Olmo, Antonio CSIC ORCID
Palabras clavePrebiotics
Isomaltooligosaccharides
Transglycosilation
Oligosaccharides
Glycosidase
Functional foods
Fecha de publicación2007
EditorElsevier
CitaciónProcess Biochemistry Volume 42, Issue 11, November 2007, Pages 1530–1536
ResumenThe transglycosylation activity of a novel alpha-glucosidase from the basidiomycetous yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) was studied using maltose as glucosyl donor. The enzyme synthesized oligosaccharides with alpha-(1-2), alpha-(1-4) and alpha-(1-6) bonds. Using 200 g/l maltose, the yield of oligosaccharides was 53.8 g/l, with prebiotic oligosaccharides containing at least one alpha-(1-6) linkage (panose, 6-O-alpha-glucosyl-maltotriose and 6-O-alpha-isomaltosyl-maltose) being the major products (47.1 g/l). The transglycosylatying yield was 3.6 times higher than the observed with the alpha-glucosidase from Saccharomyces cerevisiae (53.8 vs. 14.7 g/l). Moreover, when increasing the maltose concentration up to 525 g/l, the maximum production of tri- and tetrasaccharides reached 167.1 g/l, without altering the percentage of oligosaccharides in the mixture. Compared with other microbial alpha-glucosidases in which the main transglycosylation product is a disaccharide, the enzyme from X. dendrorhous yields a final product enriched in trisaccharides and tetrasaccharides.
Versión del editorhttp://dx.doi.org/10.1016/j.procbio.2007.08.007
URIhttp://hdl.handle.net/10261/6225
DOI10.1016/j.procbio.2007.08.007
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