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Título: | Transformation of maltose into prebiotic isomaltooligosaccharides by a novel alpha-glucosidase from Xantophyllomyces dendrorhous |
Autor: | Fernández Arrojo, Lucía; Marín Alberdi, Dolores; Gómez de Segura, María Aránzazu; Linde, Dolores CSIC ORCID ; Alcalde Galeote, Miguel CSIC ORCID ; Gutiérrez Alonso, Patricia; Ghazi, Iraj CSIC; Plou Gasca, Francisco José CSIC ORCID ; Fernández Lobato, María CSIC ORCID; Ballesteros Olmo, Antonio CSIC ORCID | Palabras clave: | Prebiotics Isomaltooligosaccharides Transglycosilation Oligosaccharides Glycosidase Functional foods |
Fecha de publicación: | 2007 | Editor: | Elsevier | Citación: | Process Biochemistry Volume 42, Issue 11, November 2007, Pages 1530–1536 | Resumen: | The transglycosylation activity of a novel alpha-glucosidase from the basidiomycetous yeast Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) was studied using maltose as glucosyl donor. The enzyme synthesized oligosaccharides with alpha-(1-2), alpha-(1-4) and alpha-(1-6) bonds. Using 200 g/l maltose, the yield of oligosaccharides was 53.8 g/l, with prebiotic oligosaccharides containing at least one alpha-(1-6) linkage (panose, 6-O-alpha-glucosyl-maltotriose and 6-O-alpha-isomaltosyl-maltose) being the major products (47.1 g/l). The transglycosylatying yield was 3.6 times higher than the observed with the alpha-glucosidase from Saccharomyces cerevisiae (53.8 vs. 14.7 g/l). Moreover, when increasing the maltose concentration up to 525 g/l, the maximum production of tri- and tetrasaccharides reached 167.1 g/l, without altering the percentage of oligosaccharides in the mixture. Compared with other microbial alpha-glucosidases in which the main transglycosylation product is a disaccharide, the enzyme from X. dendrorhous yields a final product enriched in trisaccharides and tetrasaccharides. | Versión del editor: | http://dx.doi.org/10.1016/j.procbio.2007.08.007 | URI: | http://hdl.handle.net/10261/6225 | DOI: | 10.1016/j.procbio.2007.08.007 |
Aparece en las colecciones: | (ICP) Artículos (CBM) Patentes |
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