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Title

Chemical heterogeneity in adult rat cerebellar Purkinje cells as revealed by zebrin I and low-affinity nerve growth factor receptor immunocytochemical expression following injury

AuthorsMartínez-Murillo, Ricardo ; Bentura, M. L.; Fernández García, Ana María ; Nieto-Sampedro, Manuel ; Rodrigo García, José
Issue Date1995
CitationJournal of Neurocytology 24: 807- 817 (1995)
AbstractCerebellar Purkinje cells in rat express low-affinity nerve growth factor receptor during development, but rarely in normal adult animals. However, after either mechanical injury or colchicine treatment during adulthood, these cells re-express low-affinity nerve growth factor receptor-immunoreactive protein. Two Purkinje cell subpopulations were defined in normal adult cerebellum by the presence or the absence of zebrin I antigen. Nevertheless, it remains an open question as to whether low-affinity nerve growth factor receptor-immunoreactive protein can be expressed by a]l damaged Purkinje cells, independent of their location and their staining with antibodies against intrinsic molecular markers that reveal Purkinje cell heterogeneity, such as zebrin I. In this study, a serial-section immunocytochemical mapping of the expression zebrin I and low-affinity nerve growth factor receptor, using specific monoclonal antibodies, was carried out in colchicine-treated rats. After mechanical damage of the cerebellar cortex, co-localization of these antigens at the cellular level was also analysed in thin adjacent sections, and by using a combined immunocytochemical staining method in individual sections. The findings revealed the existence of three sub-sets of Purkinje cells: (1) two complementary groups distinctly immunoreactive to one antibody, but not to the other and (2) a third group that contained double-labelled cells. In contrast, co-expression of both antigens was never observed following mechanical lesions. The seemingly independent response to mechanical injury of Purkinje cells located in different zebrin-defined compartments, indicates that particular subpopulations of Purkinje cells may respond differentially to traumatic injury.
URIhttp://hdl.handle.net/10261/62096
DOI10.1007/BF01179980
Identifiersdoi: 10.1007/BF01179980
issn: 0300-4864
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