Antioxidant activity of phenolic compounds identified in sunflower seeds

Abstract

The antioxidant activity and phenolic compound profiles of six fractions (I-VI) obtained from sunflower seed extract were studied. HPLC-MS(ESI) analysis was applied for quantitative and qualitative determination of phenolic compounds of the fractions. The antioxidant activity of the fractions was studied in terms of their ability to scavenge DPPH · and ABTS ·+ and to reduce Fe 3+/ferricyanide complex to the ferrous form and was expressed as EC 50, TEAC and reducing power values, respectively. The results of all antioxidant activity tests showed good correlations among each other and with the phenolic contents for the individual fractions. The fractions IV-VI were characterized by high antioxidant activity. 5-O-Caffeoylquinic acid was a predominant compound of fractions IV and V, while dicaffeoylquinic acid isomers and caffeoyl-dimethoxycinnamoylquinic acid isomers accounted for 76.6 % of phenolic compounds of fraction VI. Ferulic acid, p-coumaroylquinic acid isomers, ferulic acid dehydrotrimer isomers and some quercetin derivatives were also identified. The highest content of those compounds was noted in fraction III.