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The role of cofactor binding in tryptophan accessibility and conformational stability of His-tagged D-amino acid oxidase from Trigonopsis variabilis

AuthorsArroyo, Miguel; Menéndez, Margarita ; García, José Luis ; Campillo, Nuria E. ; Hormigo, Daniel; Mata, Isabel de la; Castillón, María Pilar; Acebal, Carmen
d-amino acid oxidase
secondary structure
circular dichroism
thermal stability
dissociation constant
cofactor binding
Issue DateMay-2007
CitationBBA - Biochimica et Biophysica Acta 1774 (5 ) : 556-565 (2007)
Abstractd-amino acid oxidase from Trigonopsis variabilis (TvDAAO) is a flavoenzyme with high biotechnological and industrial interest. The overexpression and purification of the apoprotein form of a recombinant His-tagged TvDAAO allowed us to go deep into the structural differences between apoenzyme and holoenzyme, and on the cofactor binding and its contribution to enzyme stability. A significant decrease in intrinsic fluorescence emission took place upon FAD binding, associated to cofactor induced conformational transitions or subunit dimerization that could affect the local environment of protein tryptophan residues. Furthermore, acrylamide-quenching experiments indicated that one of the five tryptophan residues of TvDAAO became less accessible upon FAD binding. A Kd = 1.5 ± 0.1 × 10− 7 M for the dissociation of FAD from TvDAAO was calculated from binding experiments based on both quenching of FAD fluorescence and activity titration curves. Secondary structure prediction indicated that TvDAAO is a mixed α/β protein with 8 α-helices and 14 β-sheets connected by loops. Prediction results were in good agreement with the estimates obtained by circular dichroism which indicated that both the apoenzyme and the holoenzyme had the same structural component ratios: 34% α-helix content, 20% β-structure content (14% antiparallel and 6% parallel β-sheet), 15% β-turns and 31% of random structure. Circular dichroism thermal-transition curves suggested single-step denaturation processes with apparent midpoint transition temperatures (Tm) of 37.9 °C and 41.4 °C for the apoenzyme and the holoenzyme, respectively. A three-dimensional model of TvDAAO built by homology modelling and consistent with the spectroscopic studies is shown. Comparing our results with those reported for pig kidney (pkDAAO) and Rhodotorula gracilis (RgDAAO) d-amino acid oxidases, a “head-to-head” interaction between subunits in the TvDAAO dimer might be expected
Description10 páginas, 11 figuras -- PAGS nro. 556-565
Publisher version (URL)http://dx.doi.org/10.1016/j.bbapap.2007.03.009
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