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dc.contributor.authorSayagués, José María-
dc.contributor.authorGutiérrez, María Laura-
dc.contributor.authorGonzález González, María-
dc.contributor.authorJensen, Evan-
dc.contributor.authorOrfao, Alberto-
dc.date.accessioned2012-11-21T10:57:03Z-
dc.date.available2012-11-21T10:57:03Z-
dc.date.issued2010-
dc.identifierdoi: 10.1002/path.2712-
dc.identifierissn: 0022-3417-
dc.identifiere-issn: 1096-9896-
dc.identifier.citationJournal of Pathology 221(3): 308-319 (2010)-
dc.identifier.urihttp://hdl.handle.net/10261/60735-
dc.description.abstractMuch has been learned about the chromosomal abnormalities of colorectal carcinomas but the cytogenetic relationship between the neoplastic clones present in primary versus metastatic tumour samples remains unclear. We analyse the frequency of abnormalities for 47 chromosome regions using the interphase fluorescence in situ hybridization technique in a group of 48 tumours, including 24 primary colorectal tumours and 24 paired liver metastases. All tumours showed complex karyotypes with numerical/structural abnormalities for seven or more different chromosomes/chromosome regions both in the primary tumours and in their paired metastases. Chromosome 8 was the most frequently altered (22/24 primary tumours), consistently showing del(8p22) and/or gains/amplification of 8q24, followed by abnormalities of the entire chromosome 7 (21/24 primary tumours) and of chromosomes 17p and 20q (20/24 primary tumours). Simultaneous staining for multiple chromosome probes revealed the presence of two or more tumour cell clones in 23/24 cases (46/48 tumour samples). Interestingly, the liver metastases typically contained tumour cell clones similar to those found in the primary tumours, suggesting the absence of selective selection of specific tumour clones. Despite this, additional chromosomal abnormalities were detected in 23/24 metastatic tumours, which preferentially consisted of del(17p13) and gains/amplification of 11q13 and 20q13; moreover, compared to primary tumours, metastases showed an increased number of abnormalities of chromosomes 1p, 7q, 8q, 13q, and 18q, and new chromosomal abnormalities involving chromosomes 6, 10q23, 14q32, 15q22, and 19q13. Owing to the high frequency of numerical abnormalities of the entire chromosome 7 and loss and/or gain/amplification of specific regions of chromosome 8, eg del(8p22) and/or gains/amplification of 8q24 in primary colorectal tumours with associated metastases, it is suggested that their assessment at diagnosis could be of great clinical utility for the identification of colorectal cancer patients at higher risk of developing liver metastases.-
dc.description.sponsorshipThis work was partially supported by grants from the Consejeria de Sanidad, Junta de Castilla y León, Valladolid, Spain (SAN191/SA09/06 and SAN673/SA39/ 08); Fundación Memoria de Don Samuel Solórzano Barruso, Salamanca, Spain; Caja de Burgos (Obra Social), Burgos, Spain; Grupo Excelencia de Castilla y León (GR37); and the RTICC from the Instituto de Salud Carlos III (ISCIII), Ministerio de Sanidad y Consumo, Madrid, Spain (RD06/0020/0035-FEDER). JM Sayagués and M González were supported by grants (CP05/00321 and FI08/00721, respectively) from the ISCIII, Ministerio de Ciencia e Innovación, Madrid, Spain. -
dc.language.isoeng-
dc.publisherJohn Wiley & Sons-
dc.rightsclosedAccess-
dc.titleIntratumoural cytogenetic heterogeneity of sporadic colorectal carcinomas suggests several pathways to liver metastasis-
dc.typeArtículo-
dc.identifier.doi10.1002/path.2712-
dc.date.updated2012-11-21T10:57:03Z-
dc.description.versionPeer Reviewed-
Appears in Collections:(IBMCC) Artículos
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