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Title

Differential Estrogenic Effects of the Persistent Organochlorine Pesticides Dieldrin, Endosulfan, and Lindane in Primary Neuronal Cultures

AuthorsBriz, Víctor ; Molina-Molina, José-Manuel; Sánchez-Redondo, Sara ; Fernández, Mariana F.; Grimalt, Joan O. ; Olea, Nicolás; Rodríguez-Farré, Eduard; Suñol, Cristina
KeywordsPesticides
Estrogen receptor
Protein kinase B (Akt)
Extracellular-regulated kinase
GABAA receptor
Neuronal cultures
Issue Date2011
PublisherOxford University Press
CitationToxicological Sciences 120 (2): 413-427 (2011)
AbstractThe organochlorine chemicals endosulfan, dieldrin, and ghexachlorocyclohexane (lindane) are persistent pesticides to which people are exposed mainly via diet. Their antagonism of the g-aminobutyric acid-A (GABAA) receptor makes them convulsants. They are also endocrine disruptors because of their interaction with the estrogen receptor (ER). Here, we study the effects of dieldrin, endosulfan, and lindane on ERs in primary cultures of cortical neurons (CN) and cerebellar granule cells (CGC). All the compounds tested inhibited the binding of [3H]-estradiol to the ER in both CN and CGC, with dieldrin in CGC showing the highest affinity.We also determined the effects of the pesticides on protein kinase B (Akt) and extracellular-regulated kinase 1 and 2 (ERK1/2) phosphorylation. Dieldrin and endosulfan increased Akt phosphorylation in CN, which was inhibited by the ERb antagonist 4-[2-phenyl-5,7- bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol. Instead, Akt and ERK1/2 phosphorylation induced by dieldrin in CGC was mediated by multiple activation of ERa, ERb, and G protein– coupled receptor 30. Lindane did not activate these pathways, but it inhibited estradiol-mediated Akt and ERK1/2 activation. In CN, all the chemicals activated ERK1/2 through a mechanism involving GABAA and glutamate receptors. Long-term exposure to these pesticides reduced the levels of ERa, but not of ERb. Moreover, extracts of CN treated with endosulfan, dieldrin, or lindane induced cell proliferation in MCF-7 human breast cancer–derived cells, whereas only extracts of CGC treated with dieldrin induced MCF-7 cell proliferation. Overall, the observed alterations on ER-mediated signaling and ER levels in neurons might contribute to the neurotoxicity of these organochlorine pesticides.
Publisher version (URL)http://dx.doi.org/10.1093/toxsci/kfr019
URIhttp://hdl.handle.net/10261/60696
DOI10.1093/toxsci/kfr019
ISSN1096-6080
E-ISSN1096-0929
Appears in Collections:(IDAEA) Artículos
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