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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/60660
Title: Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks
Authors: Gutiérrez Suárez, Ana ; Rencoret, Jorge ; Cadena, Edith M. ; Rico Campos, Alejandro; Barth, Dorothee; Río Andrade, José Carlos del ; Martínez, Ángel T.
Keywords: Enzymatic deconstruction
Issue Date: Sep-2012
Publisher: Elsevier
Citation: Bioresource Technology 119:114-122(2012)
Abstract: The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment
Description: 9 páginas, 6 figuras, 4 tablas -- PAGS nros. 267-277
Publisher version (URL): http://dx.doi.org/10.1016/j.biortech.2012.05.112
URI: http://hdl.handle.net/10261/60660
DOI: 10.1016/j.biortech.2012.05.112
ISSN: 0960-8524
E-ISSN: 1873-2976
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