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Título : Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks
Autor : Gutiérrez Suárez, Ana, Rencoret, Jorge, Cadena, Edith M., Rico Campos, Alejandro, Barth, Dorothee, Río Andrade, José Carlos del, Martínez Ferrer, Ángel Tomás
Palabras clave : Enzymatic deconstruction
laccase
bioethanol
lignocellulose
2D NMR
Fecha de publicación : Sep-2012
Editor: Elsevier
Resumen: The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment
Descripción : 9 páginas, 6 figuras, 4 tablas -- PAGS nros. 267-277
Versión del editor: http://dx.doi.org/10.1016/j.biortech.2012.05.112
URI : http://hdl.handle.net/10261/60660
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2012.05.112
Citación : Bioresource Technology 119:114-122(2012)
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