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Open Access item Demonstration of laccase/mediator-based removal of lignin from wood and non-wood feedstocks

Authors:Gutiérrez Suárez, Ana
Rencoret, Jorge
Cadena, Edith M.
Rico Campos, Alejandro
Barth, Dorothee
Río Andrade, José Carlos del
Martínez Ferrer, Ángel Tomás
Keywords:Enzymatic deconstruction, laccase, bioethanol, lignocellulose, 2D NMR
Issue Date:Sep-2012
Citation:Bioresource Technology 119:114-122(2012)
Abstract:The ability of Trametes villosa laccase, in conjuction with 1-hydroxybenzotriazole (HBT) as mediator and alkaline extraction, to remove lignin was demonstrated during treatment of wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) feedstocks. At 50 U g−1 laccase and 2.5% HBT concentration, 48% and 32% of the Eucalyptus and Pennisetum lignin were removed, respectively. Two-dimensional nuclear magnetic resonance of the feedstocks, swollen in dimethylsulfoxide-d6, revealed the removal of p-hydroxyphenyl, guaiacyl and syringyl lignin units and aliphatic (mainly β-O-4′-linked) side-chains of lignin, and a moderate removal of p-coumaric acid (present in Pennisetum) without a substantial change in polysaccharide cross-signals. The enzymatic pretreatment (at 25 U g−1) of Eucalyptus and Pennisetum feedstocks increased the glucose (by 61% and 12% in 72 h) and ethanol (by 4 and 2 g L−1 in 17 h) yields from both lignocellulosic materials, respectively, as compared to those without enzyme treatment
Description:9 páginas, 6 figuras, 4 tablas -- PAGS nros. 267-277
Publisher version (URL):http://dx.doi.org/10.1016/j.biortech.2012.05.112
E-ISSNmetadata.dc.identifier.doi = DOI:1873-2976
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