Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/60481
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Título : How Vav proteins discriminate the GTPases Rac1 and Rhoa from Cdc42
Autor : Movilla, Nieves, Dosil, Mercedes, Bustelo, Xosé R.
Fecha de publicación : 2001
Editor: Nature Publishing Group
Resumen: Vav proteins are GDP/GTP exchange factors for Rho/Rac GTPases that are activated by tyrosine phosphorylation. These proteins activate Rac1, RhoG, and RhoA but not the highly related Cdc42 protein. At present, there is no available information to explain this substrate selectivity at the structural level. Here we show that the selection of Vav proteins substrates is achieved at two different levels. On one hand, Var proteins utilize some residues of the β2/β3 region of Rho/Rac GTPases (D49 and E54) to assure the specific binding to its substrate. In addition, these exchange factors need a second structural signal located in the β5 region of Rho/Rac proteins (residue K118) to promote proper GDP/GTP exchange. These results identify the amino acid residues that allow the discrimination of the Var family substrates from Cdc42 and, in addition, demonstrate that the activation of specific Rho/Rac GTPases by these GEFs requires two concatenated events, binding and subsequent enzyme reaction, whose specificities are determined by two separate regions of Rho proteins.
URI : http://hdl.handle.net/10261/60481
Identificadores: doi: 10.1038/sj.onc.1205000
issn: 0950-9232
e-issn: 1476-5594
DOI: 10.1038/sj.onc.1205000
Citación : Oncogene 20(56): 8057-8065 (2001)
Appears in Collections:(IBMCC) Artículos

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