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Quantitative high-resolution 13C NMR analysis of lipids extracted from the white muscle of atlantic tuna (Thunnus alalunga)

AuthorsSacchi, Raffaele; Medina, Isabel ; Aubourg, Santiago P. ; Giudicianni, Italo; Paolillo, Livio; Addeo, Francesco
Issue Date1993
PublisherAmerican Chemical Society
CitationJournal of Agricultural and Food Chemistry 41(8): 1247-1253 (1993)
AbstractLipids extracted from albacore tuna (Thunnus alalunga) were studied using high-resolution l3C nuclear magnetic resonance (NMR) spectroscopy. Simultaneous quantitative determination of free fatty acids, lipid classes, and fatty acids CY-@ positional distribution in triacylglycerols was obtained. Glyceride and phospholipid composition was studied using glyceryl resonances. Lipids extracted from fresh white muscles of Atlantic tuna had a different triacylglycerol and phospholipid content, related to fish weight. Free fatty acid carbonyl resonances were detected at the lower field of the 13C NMR carbonyl region and used for its quantitative determination. NMR data agreed with those obtained using UV spectrophotometry, showing that NMR is a suitable method to follow lipolytic alterations. The careful study of glyceryl and carbonyl resonances also leads to the direct determination of fatty acid a-@ distribution in triacylglycerols. The quantitative analysis of fatty acid composition and distribution was then achieved, and results obtained using high-resolution NMR were in good agreement with those obtained by gas chromatography. Polyunsaturated fatty acids showed a preferential location in the @-acypl osition, whereas monounsaturated fatty acids were found preferentially esterified in the a-position. The method may be used to monitor the composition and changes in fish lipids and oils during processing or storage.
Description7 páginas, 6 tablas, 6 figuras
Publisher version (URL)http://dx.doi.org/10.1021/jf00032a016
Appears in Collections:(IIM) Artículos
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