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Closed Access item Immunizing pigs with Ascaris suum haemoglobin increases the inflammatory response in the liver but fails to induce a protective immunity
Martínez Valladares, María
|Keywords:||Ascaris suum, Haemoglobin, Vaccination, White spots|
|Citation:||Parasite Immunology 33: 250-254 (2011)|
|Abstract:||To determine whether purified Ascaris suum haemoglobin
(AsHb) is a suitable vaccine candidate for the control of
Ascaris infections, pigs were vaccinated with AsHb in combination
with QuilA adjuvant and challenged with A. suum eggs.
The number of liver lesions and worms in the intestine was
assessed on day 14, 28 and 56 post-infection (p.i.). No significant
differences were found in the number of worms recovered
between vaccinated and control pigs on any of these days.
However, significantly more white spots were counted on the
livers of vaccinated pigs on day 14 (+86%) and day 28
(+118%) p.i. compared with nonvaccinated controls. To investigate
whether the increased immunoreactivity against the
liver stage L3s in vaccinated pigs was triggered by and directed
against AsHb, the transcription and expression of AsHb in
this larval life stage was analysed by RT-PCR and immunoblotting.
The results showed that neither the AsHb transcript
nor protein was detectable in freshly hatched L3. However, the
immunoblot analysis showed that vaccination with AsHb
resulted in the production of antibodies binding to several
other antigens of the L3, suggesting that these might be
involved in the increased white spot development.|
|Description:||5 páginas, 1 figura, 1 tabla.|
|Publisher version (URL):||http://dx.doi.org/10.1111/j.1365-3024.2010.01274.x|
|Appears in Collections:||(IGM) Artículos|
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