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Título : Vesicle motion and fusion are altered in chromaffin cells with increased SNARE cluster dynamics
Autor : López-Font, Inmaculada, Ortíz, José-Antonio, Villanueva, José, Torres, Vanesa, Torregrosa-Hetland, Cristina J., Francés, María del Mar, Viniegra, Salvador, Gutiérrez, Luis M.
Fecha de publicación : 2009
Editor: Blackwell Publishing
Resumen: The expression of SNAP-25 fused to green fluorescent protein (GFP) has been instrumental in demonstrating SNARE role in exocytosis. The wild-type GFP-SNAP-25 and a Δ9 form, product of botulinum neurotoxin A activity, the main ingredient in the BOTOX preparation, were employed here to study SNARE implication in vesicle mobility and fusion in cultured bovine chromaffin cells, a neuroendocrine exocytotic model. Using total internal reflection fluorescent microscopy, we have identified membrane microdomains of 500-600nm diameter that contain both SNAP-25 and syntaxin-1 and associate with synaptobrevin-2. Interestingly, while the SNAP-25 Δ9 formed similar clusters, they displayed increased mobility both laterally and in the axis perpendicular to the plasmalemma, and this correlates with the enhanced dynamics of associated chromaffin granules. SNARE cluster-enhanced motion is reversed by elevation of the intracellular calcium level. Furthermore, single vesicle fusion was unlikely in the highly mobile vesicles present in the cells expressing SNAP-25 Δ9, which, in addition, displayed in average slower fusion kinetics. Consequently, SNARE cluster dynamics is a new aspect to consider when determining the factors contributing to the mobility of the vesicles in close vicinity to the plasma membrane and also the probability of exocytosis of this granule population.
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Identificadores: doi: 10.1111/j.1600-0854.2008.00861.x
issn: 1398-9219
e-issn: 1600-0854
Citación : Traffic 10(2): 172-185 (2009)
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