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Title

Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus

AuthorsCosta, M. M. ; Pereiro, Patricia ; Wang, T.; Secombes, Christopher J.; Figueras Huerta, Antonio ; Novoa, Beatriz
KeywordsIL-22
IL-17
Th-17 response
Mucosal-associated immunity
Issue Date2012
PublisherElsevier
CitationDevelopmental and Comparative Immunology 38(4): 505-516 (2012)
AbstractThis report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals.
Description12 páginas, 8 figuras, 3 tablas
Publisher version (URL)http://dx.doi.org/10.1016/j.dci.2012.09.002
URIhttp://hdl.handle.net/10261/59774
DOI10.1016/j.dci.2012.09.002
ISSN0145-305X
E-ISSN1879-0089
Appears in Collections:(IIM) Artículos
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