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Open Access item Characterization and gene expression analysis of the two main Th17 cytokines (IL-17A/F and IL-22) in turbot, Scophthalmus maximus

Authors:Costa, M. M.
Pereiro, Patricia
Wang, T.
Secombes, Christopher J.
Figueras, Antonio
Novoa, Beatriz
Keywords:IL-22, IL-17, Th-17 response, Mucosal-associated immunity
Issue Date:2012
Publisher:Elsevier
Citation:Developmental and Comparative Immunology 38(4): 505-516 (2012)
Abstract:This report describes the cloning, characterization and gene expression pattern of two Th17 cytokines, interleukin (IL)-17A/F and -22, in turbot Scophthalmus maximus. The turbot IL-17A/F cDNA contains a 516 bp open reading frame encoding a deduced IL-17A/F protein of 171 amino acid (aa) residues, containing a predicted signal peptide of 31 aa. Turbot IL-22 had a 564 bp ORF coding for a 187 aa protein with a 33 aa signal peptide. The turbot IL-22 protein contained a typical IL-10 family signature. Both cytokines had highest expression levels in the intestine followed by head kidney and gills. Stimulation with the Gram negative bacterium Aeromonas salmonicida was able to modulate IL-17A/F and IL-22 expression in head kidney, spleen and liver but not the intestine. PMA and PHA were also able to induce the expression of both cytokines, suggesting that, as expected, T-cells are likely the main producers of these molecules in turbot as in mammals.
Description:12 páginas, 8 figuras, 3 tablas
Publisher version (URL):http://dx.doi.org/10.1016/j.dci.2012.09.002
URI:http://hdl.handle.net/10261/59774
ISSN:0145-305X
E-ISSNmetadata.dc.identifier.doi = DOI:1879-0089
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Appears in Collections:(IIM) Artículos

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