English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/5944
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

cDNA-AFLP analysis of seed germination in Arabidopsis thaliana identifies transposons and new genomic sequences

AuthorsGutiérrez de Diego, Juana; Rodríguez García, David; Rodríguez Lorenzo, José Luis; Grappin, Philippe; Cervantes, Emilio
KeywordsArabidopsis
cDNA-AFLP
Germination
Gibberellic acid
Transposon
Issue DateMar-2006
PublisherElsevier
CitationJournal of Plant Physiology 163(4): 452-462 (2006)
AbstractA cDNA-AFLP experiment was designed to identify and clone nucleotide sequences induced during seed germination in Arabidopsis thaliana. Sequences corresponding to known genes involved in processes important for germination, such as mitochondrial biogenesis, protein synthesis and cell cycle progression, were isolated. Other sequences correspond to Arabidopsis BAC clones in regions where genes have not been annotated. Notably, a number of the sequences cloned did not correspond to available sequences in the databases from the Arabidopsis genome, but instead present significant similarity with DNA from other organisms, for example fish species; among them, some may encode transposons. A number of the sequences isolated showed no significant similarity with any sequences in the public databases. Oligonucleotides derived from these new sequences were used to amplify genomic DNA of Arabidopsis. Expression analysis of representative sequences is presented. This work suggests that, during germination, there may be a massive transposon mobilization that may be useful in the annotation of new genome sequences and identification of regulatory mechanisms.
Description11 pages, 4 figures.-- PMID: 16455359 [PubMed].-- Available online Aug 3, 2005.
Publisher version (URL)http://dx.doi.org/10.1016/j.jplph.2005.04.032
URIhttp://hdl.handle.net/10261/5944
DOI10.1016/j.jplph.2005.04.032
ISSN0176-1617
Appears in Collections:(IRNASA) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.