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Título : Fine-tuning tomato agronomic properties by computational genome redesign
Autor : Carrera, Javier, Fernández-del-Carmen, Asun, Fernández-Muñoz, Rafael, Rambla, JoséLuis, Pons, Clara, Jaramillo, Alfonso, Elena, Santiago F., Granell, Antonio
Palabras clave : Transcriptional control
Line population
Fecha de publicación : Jun-2012
Editor: Public Library of Science
Resumen: Considering cells as biofactories, we aimed to optimize its internal processes by using the same engineering principles that large industries are implementing nowadays: lean manufacturing. We have applied reverse engineering computational methods to transcriptomic, metabolomic and phenomic data obtained from a collection of tomato recombinant inbreed lines to formulate a kinetic and constraint-based model that efficiently describes the cellular metabolism from expression of a minimal core of genes. Based on predicted metabolic profiles, a close association with agronomic and organoleptic properties of the ripe fruit was revealed with high statistical confidence. Inspired in a synthetic biology approach, the model was used for exploring the landscape of all possible local transcriptional changes with the aim of engineering tomato fruits with fine-tuned biotechnological properties. The method was validated by the ability of the proposed genomes, engineered for modified desired agronomic traits, to recapitulate experimental correlations between associated metabolites.
Descripción : This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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ISSN: 1553-7358
Citación : Plos Computational Biology 8(6): e1002528 (2012)
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