Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/59092
Título : Transcriptomics in human blood incubation reveals the importance of oxidative stress response in Saccharomyces cerevisiae clinical strains
Autor : Llopis, Silvia, Querol, Amparo, Heyken, Antje, Hube, Bernhard, Jespersen, Lene, Fernández-Espinar, M T, Pérez-Torrado, Roberto
Fecha de publicación : 23-Aug-2012
Editor: BioMed Central
Citación : BMC Genomics. 13(1):419 (2012)
Resumen: Abstract Background In recent years an increasing number of yeast infections in humans have been related to certain clinical isolates of Saccharomyces cerevisiae. Some clinical strains showed in vivo and in vitro virulence traits and were able to cause death in mice whereas other clinical strains were avirulent. Results In this work, we studied the transcriptional profiles of two S. cerevisiae clinical strains showing virulent traits and two control non-virulent strains during a blood incubation model and detected a specific transcriptional response of clinical strains. This response involves an mRNA levels increase of amino acid biosynthesis genes and especially oxidative stress related genes. We observed that the clinical strains were more resistant to reactive oxygen species in vitro. In addition, blood survival of clinical isolates was high, reaching similar levels to pathogenic Candida albicans strain. Furthermore, a virulent strain mutant in the transcription factor Yap1p, unable to grow in oxidative stress conditions, presented decreased survival levels in human blood compared with the wild type or YAP1 reconstituted strain. Conclusions Our data suggest that this enhanced oxidative stress response in virulent clinical isolates, presumably induced in response to oxidative burst from host defense cells, is important to increase survival in human blood and can help to infect and even produce death in mice models.
URI : http://hdl.handle.net/10261/59092
Identificadores: http://dx.doi.org/10.1186/1471-2164-13-419
Citación : BMC Genomics. 13(1):419 (2012)
Appears in Collections:(IATA) Artículos

Files in This Item:
File Description SizeFormat 
1471-2164-13-419.xml106,25 kBXMLView/Open
1471-2164-13-419.pdf513,4 kBAdobe PDFView/Open
1471-2164-13-419-S2.XLSX568,03 kBMicrosoft Excel XMLView/Open
1471-2164-13-419-S1.DOCX23,72 kBMicrosoft Word XMLView/Open
Show full item record
 
CSIC SFX LinksSFX Query

Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.