Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/59052
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A new protein carrying an NmrA-like domain is required for cell differentiation and development in Dictyostelium discoideum |
Autor: | Núñez-Corcuera, Beatriz CSIC ORCID; Serafimidis, Ioannis; Arias-Palomo, Ernesto CSIC ORCID ; Rivera-Calzada, Angel CSIC ORCID; Suárez, Teresa CSIC ORCID | Palabras clave: | Dictyostelium Prestalk A cells Ammonia NmrA Ammonium transporters Pattern formation |
Fecha de publicación: | 15-sep-2008 | Editor: | Elsevier | Citación: | Developmental Biology 321(2):331-342(2008) | Resumen: | We have isolated a Dictyostelium mutant unable to induce expression of the prestalk-specific marker ecmB in monolayer assays. The disrupted gene, padA, leads to a range of phenotypic defects in growth and development. We show that padA is essential for growth, and we have generated a thermosensitive mutant allele, padA−. At the permissive temperature, mutant cells grow poorly; they remain longer at the slug stage during development and are defective in terminal differentiation. At the restrictive temperature, growth is completely blocked, while development is permanently arrested prior to culmination. padA− slugs are deficient in prestalk A cell differentiation and present an abnormal ecmB expression pattern. Sequence comparisons and predicted three-dimensional structure analyses show that PadA carries an NmrA-like domain. NmrA is a negative transcriptional regulator involved in nitrogen metabolite repression in Aspergillus nidulans. PadA predicted structure shows a NAD(P)+-binding domain, which we demonstrate that is essential for function. We show that padA− development is more sensitive to ammonia than wild-type cells and two ammonium transporters, amtA and amtC, appear derepressed during padA− development. Our data suggest that PadA belongs to a new family of NAD(P)+-binding proteins that link metabolic changes to gene expression and is required for growth and normal developmen | Descripción: | 12 páginas, 10 figuras, 1 tabla, 3 figuras suplementarias -- PAGS nros. 331-342 | Versión del editor: | http://dx.doi.org/10.1016/j.ydbio.2008.06.027 | URI: | http://hdl.handle.net/10261/59052 | DOI: | 10.1016/j.ydbio.2008.06.027 | ISSN: | 0012-1606 |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
restringido.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
16
checked on 01-abr-2024
WEB OF SCIENCETM
Citations
12
checked on 21-feb-2024
Page view(s)
356
checked on 19-abr-2024
Download(s)
232
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.