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Closed Access item Determination of cobalamins (hydroxo-, cyano-, adenosyl- and methyl-cobalamins) in seawater using reversed-phase liquid chromatography with diode-array detection
|Citation:||Analytica Chimica Acta 701: 81- 85 (2011)|
|Abstract:||A high-performance liquid chromatography method was developed for the separation and determination of four cobalamins in seawater. Chromatographic separation was performed on a reversed-phase discovery RP-amide C16 column with buffer potassium dihydrogenphosphate and acetonitrile as the mobile phases in linear gradients elution mode. Cobalamins were previously preconcentrated in C18 resins cartridges. Detection was performed using UV-diode array detector in a range of λ of 200-400nm. The method showed to be linear over a range of 1-300ngmL-1 with acceptable precision and accuracy. The detection limits ranged between 0.07pgmL-1 for 5′-deoxyadenosylcobalamin and 0.5pgmL-1 for hydroxocobalamin. The mean cobalamins recoveries for direct determination ranged between 76 and 93% for hydroxo-, cyano- and methylcobalamin, while the recovery for 5′-deoxyadenosylcobalamin was only 31% suggesting that the preconcentration method was not valid for this cobalamin. The method was successfully applied to coastal seawater where the concentrations ranged from 4.2 to 7.3ngL-1 for hydroxo-, 1.4-3.9ngL-1 for cyano-, 2.1-4.6ngL-1 for 5′-deoxyadenosyl- and 33-83.5ngL-1 for methylcobalamin. © 2011 Elsevier B.V.|
|Appears in Collections:||(IMEDEA) Artículos|
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