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Closed Access item Early attack and subsequent changes produced in an industrial lignin by a fungal laccase and a laccase-mediator system: an analytical approach

Authors:González Arzola, K.
Polvillo, Oliva
Arias Fernández, Mª E.
Perestelo, F.
Carnicero, A.
González-Vila, Francisco Javier
Falcón, M. A.
Issue Date:2006
Citation:Applied Microbiology and Biotechnology 73(1): 141- 150 (2006)
Abstract:An industrial kraft pine lignin (Indulin AT, KL) was characterized and treated in both aqueous-buffered media and dioxane to water, either with a partially purified laccase from Fusarium proliferatum or with the laccase plus 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid (ABTS) as mediator. The changes in the lignin after different incubation periods were analyzed through the application of high performance liquid chromatography (HPLC), UV-visible (Vis) spectroscopy and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). At the onset of incubation, laccase-treated samples showed a slight polymerization and strong modifications in UV-Vis spectra. Through Py-GC/MS, a decrease in phenolic and methoxy-bearing pyrolysis products was observed, in contrast to an increase in the more oxidized products. After longer incubation periods (48 h) a substantial polymerization was detected by HPLC, along with a decrease in the guaiacyl (G) units. In contrast, the analysis by HPLC of the samples recovered from the laccase-ABTS system (LMS) showed an intense depolymerization, accompanied by a sizeable loss in G units and a decrease in the methyl and ethyl side-chain phenolic compounds. These results provide conclusive evidence of a rapid initial attack of the industrial lignin by laccase and notable modifications in the KL after longer incubation periods with laccase or LMS. © 2006 Springer-Verlag.
Identifiers:doi: 10.1007/s00253-006-0630-0
issn: 0175-7598
e-issn: 1432-0614
Appears in Collections:(IRNAS) Artículos

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