English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/57925
Title: Neurotoxicity Induced by Okadaic Acid in the Human Neuroblastoma SH-SY5Y Line Can Be Differentially Prevented by α7 and β2* Nicotinic Stimulation
Authors: Barrio, Laura del; Martín-de-Saavedra, María Dolores; Romero, Alejandro ; Parada, Esther; Egea, Javier; Ávila, Jesús; McIntosh, John Michael; Wonnacott, Susan; López, Manuela G.
Keywords: Okadaic acid
SH-SY5Y neuroblastoma
Nicotinic receptors
Hyperphosphorylation of tau
PNU 282987
5IA 85380
Issue Date: 2011
Publisher: Oxford University Press
Citation: Toxicological Sciences 123 (1): 193-205 (2011)
Abstract: A good model of neuronal death that reproduces the characteristic tau (τ) hyperphosphorylation of Alzheimeŕs disease is the use of okadaic acid (OA). The aim of this study was to determine the contribution of α7 and β2* nicotinic acetylcholine receptor (nAChR) subtypes to neuroprotection against OA in the SH-SY5Y cell line by using the selective α7 and β2* nAChR agonists PNU 282987 and 5-Iodo-A85380, respectively. The results of this study show that both α7 and β2* nAChR can afford neuroprotection against OA-induced neurotoxicity. Protection mediated by α7 nAChRs was independent of Ca2+ and involved the intracellular signaling pathway Janus Kinase-2/Phosphatidylinositol-3-kinase/Akt. When Ca2+ entry was promoted through the α7 nAChR by using the α7-selective positive allosteric modulator PNU 120596, protection was lost. By contrast, protection mediated by β2* nAChRs was Ca2+ dependent and implicated the signaling pathways PI3K/Akt and extracellular regulated kinase 1/2. Both α7 and β2* nAChR activation converged on downregulation of GSK-3β and reduction of τ phosphorylation in cells undergoing cell death induced by OA. Therefore, targeting nAChR could offer a strategy for reducing neurodegeneration secondary to hyperphosphorylation of protein τ.
Publisher version (URL): http://dx.doi.org/10.1093/toxsci/kfr163
URI: http://hdl.handle.net/10261/57925
DOI: 10.1093/toxsci/kfr163
ISSN: 1096-6080
E-ISSN: 1096-0929
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.