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Open Access item Iron-regulated metabolites produced by Pseudomonas fluorescens WCS374r are not required for eliciting induced systemic resistance against Pseudomonas syringae pv. tomato in Arabidopsis
Van Loon, Leendert C.
Bakker, Peter A. H. M.
|Keywords:||Induced systemic resistance, Pseudobactin374, Pseudomonine, Salicylic acid|
|Citation:||MicrobiologyOpen 2012; 1(3): 311–325|
|Abstract:||The plant growth-promoting rhizobacterium Pseudomonas fluorescens WCS374r
produces several iron-regulated metabolites, including the fluorescent siderophore
pseudobactin (Psb374), salicylic acid (SA), and pseudomonine (Psm), a
siderophore that contains a SA moiety. After purification of Psb374 from culture
supernatant of WCS374r, its structure was determined following isoelectrofocusing
and tandem mass spectrometry, and found to be identical to the
fluorescent siderophore produced by P. fluorescens ATCC 13525. To study the
role of SA and Psm production in colonization of Arabidopsis thaliana roots
and in induced systemic resistance (ISR) against Pseudomonas syringae pv.
tomato (Pst) by strain WCS374r, mutants disrupted in the production of these
metabolites were obtained by homologous recombination. These mutants were
further subjected to transposon Tn5 mutagenesis to generate mutants also
deficient in Psb374 production. The mutants behaved similar to the wild type
in both their Arabidopsis rhizosphere-colonizing capacity and their ability to
elicit ISR against Pst. We conclude that Psb374, SA, and Psm production by
P. fluorescens WCS374r are not required for eliciting ISR in Arabidopsis.|
|Description:||16 pages; 5 figures; 4 tables|
|Publisher version (URL):||http.//dx.doi.org/10.1002/mbo3.32|
|E-ISSNmetadata.dc.identifier.doi = DOI:||2045-8827|
|Appears in Collections:||(IAS) Artículos|
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