Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/57471
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Araújo-Bazán, Lidia | - |
dc.contributor.author | Fernández-Martínez, Javier | - |
dc.contributor.author | Ríos, Vivian de los | - |
dc.contributor.author | Etxebeste, Oier | - |
dc.contributor.author | Albar, Juan Pablo | - |
dc.contributor.author | Peñalva, Miguel Ángel | - |
dc.contributor.author | Espeso, Eduardo A. | - |
dc.date.accessioned | 2012-10-08T10:01:34Z | - |
dc.date.available | 2012-10-08T10:01:34Z | - |
dc.date.issued | 2008-03 | - |
dc.identifier.citation | Fungal Genetics and Biology 45(3):278-291(2008) | es_ES |
dc.identifier.issn | 1087-1845 | - |
dc.identifier.uri | http://hdl.handle.net/10261/57471 | - |
dc.description | 14 páginas, 7 figuras, 4 figuras suplementarias, 1 tabla, 1 tabla suplementaria -- PAGS nros. 278-291 | es_ES |
dc.description.abstract | In eukaryotic cells, importin α is the major carrier for transport protein cargoes into the nucleus. We characterize here kapA, the single Aspergillus nidulans gene encoding an importin α. Using an affinity approach, we identify six potential interactors of KapA50, a deleted version of KapA lacking the autoinhibitory importin-beta-binding domain. One such interactor is NapB, the A. nidulans orthologue of Saccharomyces cerevisiae Vps75p, a histone chaperone member of the Nap/SET family of proteins that additionally plays a cytosolic role in vacuolar protein sorting. NapB, but not its close relative NapA (the A. nidulans orthologue of yeast Nap1p) interacts directly with KapA50 in pull down assays, despite the fact that NapB does not contain a classical nuclear localization sequence. NapB is a nuclear protein which exits nuclei at the onset of mitosis when two simultaneous mechanisms might be acting, the partial disassembly of the nuclear pore complexes and as yet unidentified posttranslational modification of NapB. The mitotic cytosolic localization of NapB might facilitate its putative role in the sorting of protein cargoes to the vacuole. In addition, we show that NapB and the mitotic B-type cyclin NimE compete for in vitro binding to KapA | es_ES |
dc.description.sponsorship | We thank Dr. Dirk Görlich for plasmid pQE80zz, Dr. Stephen Osmani for plasmid pGFP and help with confocal microscopy, J.C. Sánchez for help with the phylogenic analysis, Prof. Herbert N. Arst Jr. for critical reading of the manuscript, Leire Paz for kapG cDNA cloning and E. Reoyo for technical assistance, and the Ministerio de Ciencia y Tecnología for support through Grants BFU2006-04185 to E.A.E. and BIO2006-0556 to M.A.P. J.F.-M. and L.A.-B. held a PFPI and a FPU fellowship, respectively | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | closedAccess | es_ES |
dc.subject | Aspergillus nidulans | es_ES |
dc.subject | Fungi | es_ES |
dc.subject | Importin α | es_ES |
dc.subject | Nucleosome assembly proteins | es_ES |
dc.subject | Nuclear transport | es_ES |
dc.title | NapA and NapB are the Aspergillus nidulans Nap/SET family members and NapB is a nuclear protein specifically interacting with importin alpha | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.fgb.2007.08.003 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.fgb.2007.08.003 | es_ES |
dc.identifier.e-issn | 1096-0937 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (CIB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
restringido.pdf | 21,67 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
13
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
12
checked on 28-feb-2024
Page view(s)
306
checked on 19-abr-2024
Download(s)
205
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.