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Título: | Development of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis reference method for the analysis and identification of fish species in raw and heat-processed samples: A collaborative study |
Autor: | Piñeiro, Carmen CSIC ORCID; Barros-Velázquez, Jorge CSIC ORCID; Pérez Martín, Ricardo Isaac CSIC ORCID | Palabras clave: | Electrophoresis Fish Identification Protein Species |
Fecha de publicación: | 1999 | Editor: | Wiley-VCH | Citación: | Electrophoresis 20(7): 1425-1432 (1999) | Resumen: | A collaborative study was carried out in seven European labs with the aim of achieving a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard operation procedure to identify fish species in raw and cooked samples. Urea and SDS-containing solutions were evaluated as extractants. Several preelectrophoretic operations — such as treatment with RNase/DNase, ultrafiltration and desalting — and up to ten types of gels and three SDS-PAGE systems were considered. The SDS-containing solution allowed a higher protein extractability than urea. Unlike urea extraction, SDS extraction seemed not to be influenced so much by the state of the sample (raw, cooked at 60oC, cooked at 85oC). Desalting, ultrafiltration or treatment with RNase/DNase did not improve the discriminatory power of the protein patterns. Commercial homogeneous 15% ExcelGels, especially when they were silver stained, yielded good results and afforded higher reproducibility, thus allowing a better matching of results among the laboratories participating in this collaborative study. Under the optimized technical conditions described above, all the fish species tested, either raw and cooked, yielded reproducible and discriminant species-specific protein patterns. | Descripción: | 8 páginas, 5 figuras, 1 tabla | Versión del editor: | http://dx.doi.org/10.1002/(SICI)1522-2683(19990601)20:7<1425::AID-ELPS1425>3.0.CO;2-R | URI: | http://hdl.handle.net/10261/57437 | DOI: | 10.1002/(SICI)1522-2683(19990601)20:7<1425::AID-ELPS1425>3.0.CO;2-R | ISSN: | 0173-0835 | E-ISSN: | 1522-2683 |
Aparece en las colecciones: | (IIM) Artículos |
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