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Title

Production of leukotriene B4 and prostaglandin E2 by turbot (Scophthalmus maximus) leukocytes

AuthorsTafalla, Carolina; Medina, Isabel ; Figueras Huerta, Antonio ; Novoa, Beatriz
KeywordsLeukocyte
Fish
Eicosanoids
HPLC
Leukotriene B4
Prostaglandin E2
Macrophage
Scophthalmus maximus
Issue Date1999
PublisherElsevier
CitationComparative Biochemistry and Physiology - B - Biochemistry and Molecular Biology 123(4): 351-356 (1999)
AbstractProduction of two eicosanoids derived from lipoxygenase and cyclooxygenase activities: leukotriene B4 (LTB4) and prostaglandin E2 (PGE2), respectively, have been simultaneously determined in turbot (Scophthalmus maximus) blood leucocyte and kidney macrophage supernatants by a reverse phase high performance liquid chromatography (HPLC) system coupled with a Diode–Array detector. Levels of LTB4 after calcium ionophore challenge were 4.08 ng ml 1 in blood leukocyte supernatants and 0.25 ng ml 1 in kidney macrophage supernatants. The levels found for PGE2 were 428.23 and 606.67 ng ml 1 in blood leukocytes and kidney macrophage supernatants, respectively. When blood leukocytes were treated with the respective inhibitors for the enzymes implicated on the synthesis of both compounds an inhibition of 90.35% was observed for PGE2 and 76.44% for LTB4. The detection limit of the method was 0.15 ng ml 1 for LTB4 and 50 ng ml 1 for PGE2.
Description6 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1016/S0305-0491(99)00075-9
URIhttp://hdl.handle.net/10261/57351
DOI10.1016/S0305-0491(99)00075-9
ISSN1096-4959
Appears in Collections:(IIM) Artículos
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