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dc.contributor.authorGarces, Fernando-
dc.contributor.authorFernández, Francisco J.-
dc.contributor.authorGómez, Ana M.-
dc.contributor.authorPérez-Luque, Rosa-
dc.contributor.authorCampos, E.-
dc.contributor.authorProhens, R.-
dc.contributor.authorAguilar, Juan-
dc.contributor.authorBaldomá, Laura-
dc.contributor.authorColl, Miquel-
dc.contributor.authorBadía, Josefa-
dc.contributor.authorVega, María Cristina-
dc.identifier.citationBiochemistry 47(44):11424-11433(2008)es_ES
dc.description10 páginas, 6 figuras, 2 tablas -- PAGS nros. 11424-11433es_ES
dc.description.abstractUlaR is a DNA binding protein of the DeoR family of eubacterial transcriptional repressors which maintains the utilization of the L-ascorbate ula regulon in a repressed state. The availability of L-ascorbate in the growth medium releases UlaR-mediated repression on the ula regulon, thereby activating transcription. The molecular details of this induction by L-ascorbate have remained elusive to date. Here we have identified L-ascorbate 6-phosphate as a direct effector of UlaR; using a combination of site-directed mutagenesis, gel retardation, isothermal titration calorimetry, and analytical ultracentrifugation studies, we have identified the key amino acid residues that mediate L-ascorbate 6-phosphate binding and constructed the first model of regulation of a DeoR family member, establishing the basis of the ula regulon transcription control by UlaR. In this model, specific quaternary rearrangements of the DeoR-type repressor are the molecular underpinning of the activating and repressing forms. A DNA-bound UlaR tetramer establishes repression, whereas an L-ascorbate-6-phosphate-induced breakdown of the tetrameric configuration in favor of an UlaR dimeric state results in dissociation of UlaR from DNA and allows transcription of ulaG and ula ABCDEF structural genes. Despite the fact that similar changes have been described for other unrelated repressor factors, this is the first report to demonstrate that specific oligomerization changes are responsible for the activating and repressing forms of a DeoR-type eubacterial transcriptional repressores_ES
dc.description.sponsorshipThis work was supported by grants from the Spanish Ministry of Science and Education (MEC) (BFU2006-15573/BMC to M.C.V., BFU2005-06758/BMC to M.C., and BFU2007−63090/BMC to L.B.) and from the Generalitat de Catalunya (2005SGR-00280 to M.C.). F.G. was the recipient of a predoctoral fellowship from the Generalitat de Catalunya. F.J.F. was supported by an I3P postdoctoral contract from MECes_ES
dc.publisherAmerican Chemical Societyes_ES
dc.titleQuaternary structural transitions of the UlaR repressor regulate transcriptional readout from the L-ascorbate utilization operon in Escherichia colies_ES
dc.description.peerreviewedPeer reviewedes_ES
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