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New insights into the BzdR-mediated transcriptional regulation of the anaerobic catabolism of benzoate in Azoarcus sp. CIB

AuthorsDurante-Rodríguez, Gonzalo ; Zamarro, María Teresa ; García, José Luis ; Díaz, Eduardo ; Carmona, Manuel
RNA polymerase
Issue Date1-Jan-2008
PublisherSociety for General Microbiology
CitationMicrobiology 154(1) : 306-316(2008)
AbstractThe expression of the bzd genes involved in the anaerobic degradation of benzoate in Azoarcus sp. CIB is controlled by the specific BzdR transcriptional repressor at the PN promoter. This catabolic promoter is also subject to catabolite repression by some organic acids. In vivo and in vitro experiments have shown that BzdR behaves as a repressor of the PR promoter by overlapping the transcription initiation site as well as the −35 and −10 boxes, benzoyl-CoA being the inducer molecule. In addition, by using a PN : : lacZ fusion both in Azoarcus sp. CIB and in an isogenic strain lacking the bzdR gene, we have shown that the succinate-dependent catabolite repression requires participation of the BzdR repressor
Description11 páginas, 6 figuras, 1 tabla -- PAGS nros. 306-316
Publisher version (URL)http://dx.doi.org/10.1099/mic.0.2007/011361-0
Appears in Collections:(CIB) Artículos
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