English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/57197
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

A sensitive trimethylamine-N-oxide aldolase assay in two steps without deproteinisation

AuthorsNielsen, Michael K.; Havemeister, Wiebke; Rehbein, Hartmut; González Sotelo, Carmen ; Jørgensen, Bo
KeywordsTMAOase
Enzyme assay
Fish enzyme
Formaldehyde
Issue Date2000
PublisherWiley-Blackwell
CitationJournal of the Science of Food and Agriculture 80(2): 197-200 (2000)
AbstractA two-step assay for trimethylamine-N-oxide aldolase (the target enzyme) is described in which the second step, the indicator reaction, is of the enzymatic end-point type. This indicator reaction is carried out at a slightly alkaline pH, outside the target enzyme's active pH range. An effective inactivation of the target enzyme is thus obtained solely by shifting the pH to that of the indicator reaction, thereby avoiding a deproteinisation step. By inserting a downward pH step as the stopping method, many samples may be collected and applied to the indicator reaction simultaneously. These features make the method very productive and easy to perform
Description4 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1002/(SICI)1097-0010(20000115)80:2<197::AID-JSFA513>3.0.CO;2-U
URIhttp://hdl.handle.net/10261/57197
DOI10.1002/(SICI)1097-0010(20000115)80:2<197::AID-JSFA513>3.0.CO;2-U
ISSN0022-5142
E-ISSN1097-0010
Appears in Collections:(IIM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.