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A sensitive trimethylamine-N-oxide aldolase assay in two steps without deproteinisation

AuthorsNielsen, Michael K.; Havemeister, Wiebke; Rehbein, Hartmut; González Sotelo, Carmen ; Jørgensen, Bo
Enzyme assay
Fish enzyme
Issue Date2000
CitationJournal of the Science of Food and Agriculture 80(2): 197-200 (2000)
AbstractA two-step assay for trimethylamine-N-oxide aldolase (the target enzyme) is described in which the second step, the indicator reaction, is of the enzymatic end-point type. This indicator reaction is carried out at a slightly alkaline pH, outside the target enzyme's active pH range. An effective inactivation of the target enzyme is thus obtained solely by shifting the pH to that of the indicator reaction, thereby avoiding a deproteinisation step. By inserting a downward pH step as the stopping method, many samples may be collected and applied to the indicator reaction simultaneously. These features make the method very productive and easy to perform
Description4 páginas, 4 figuras
Publisher version (URL)http://dx.doi.org/10.1002/(SICI)1097-0010(20000115)80:2<197::AID-JSFA513>3.0.CO;2-U
Appears in Collections:(IIM) Artículos
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