Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/5718
Title: Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production
Authors: Fernández García, María, Linares, Daniel M., Río Lagar, Beatriz del, Martín, M. Cruz, Álvarez González, Miguel Ángel
Keywords: Histamine detection
Lactic Acid Bacteria
Cheese
Real-Time Quantitative Polymerase Chain Reaction
Issue Date: 2006
Publisher: American Dairy Science Association
Abstract: Biogenic amines are toxic substances that appear in foods and beverages as a result of AA decarboxylation. The enzyme histidine decarboxylase catalyzes the decarboxylation of histidine to histamine, the biogenic amine most frequently involved in food poisoning. The aim of the present work was to develop a real-time quantitative PCR assay for the direct detection and quantification of histamine-producing strains in milk and cheese. A set of primers was designed, based on the histidine decarboxylase gene sequence of different gram-positive bacteria. The results show the proposed procedure to be a rapid (total processing time < 2 h), specific and highly sensitive technique for detecting potential histamine-producing strains. Chromatographic methods (HPLC) verified the capacity of real-time quantitative PCR to correctly quantify histamine accumulation.
Description: 7 pages, 1 figure.-- PMID: 16960050 [PubMed].-- Copyright © American Dairy Science Association, 2006.
Publisher version (URL): http://jds.fass.org/cgi/content/full/89/10/3763
URI: http://hdl.handle.net/10261/5718
ISSN: 0022-0302
Citation: J. Dairy Sci. 89(10): 3763-3769 (2006)
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