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dc.contributor.authorSánchez-Luque, Francisco J.-
dc.contributor.authorLópez López, Manuel Carlos-
dc.contributor.authorMacías, Francisco-
dc.contributor.authorAlonso, Carlos-
dc.contributor.authorThomas, María del Carmen-
dc.identifier.citationNucleic Acids Research 39 (18): 8065-8077 (2011)es_ES
dc.description.abstractL1Tc is a non-LTR LINE element from Trypanosoma cruzi that encodes its transposition machinery and bears an internal promoter. Herewith, we report the identification of an in vitro active hepatitis delta virus-like ribozyme located in the first 77 nt at the 5′-end of the L1Tc mRNA (L1TcRz). The data presented show that L1TcRz has a co-transcriptional function. Using gel-purified uncleaved RNA transcripts, the data presented indicate that the kinetics of the self-cleaving, in a magnesium-dependent reaction, fits to a two-phase decay curve. The cleavage point identified by primer extension takes place at +1 position of the element. The hydroxyl nature of the 5′-end of the 3′-fragment generated by the cleavage activity of L1TcRz was confirmed. Since we have previously described that the 77-nt long fragment located at the 5′-end of L1Tc has promoter activity, the existence of a ribozyme in L1Tc makes this element to be the first described non-LTR retroelement that has an internal promoter–ribozyme dual function. The L1Tc nucleotides located downstream of the ribozyme catalytic motif appear to inhibit its activity. This inhibition may be influenced by the existence of a specific L1Tc RNA conformation that is recognized by RNase P.es_ES
dc.description.sponsorshipPlan Nacional I+D+I del Ministerio de Ciencia e Innovación (MICINN)—Spain (grant numbers BFU2007/65095/BMC, BFU2007-64999/BMC, BFU2010-16470); Instituto de Salud Carlos III (ISCIII)—Redes Temáticas de Investigación Cooperativa en Salud (RETIC)—Spain (grant numbers RD06/0021/0014, RD06/0021/0008) and Fondo Europeo de Desarrollo Regional (FEDER). Funding for open access charge: Plan Nacional I+D+I del Ministerio de Ciencia e Innovación (MICINN)- Spain (grant number BFU2010-16470)es_ES
dc.publisherOxford University Presses_ES
dc.subjectTrypanosoma cruzies_ES
dc.titleIdentification of an hepatitis delta virus-like ribozyme at the mRNA 5′-end of the L1Tc retrotransposon from Trypanosoma cruzies_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderEuropean Commission-
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