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| Título: | Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing |
Autor: | Rehbein, Hartmut; Kündiger, R.; Piñeiro, Carmen CSIC ORCID; Pérez Martín, Ricardo Isaac CSIC ORCID | Palabras clave: | Parvalbumins Isoelectric focusing Marker proteins |
Fecha de publicación: | 2000 | Editor: | John Wiley & Sons | Citación: | Electrophoresis 21(8): 1458-1463 (2000) | Resumen: | An isoelectric point (pI) calibration kit containing fish muscle parvalbumins was prepared and tested for its suitability for isoelectric focusing (IEF) in the presence of 8 M urea. The pattern obtained by urea CleanGel IEF consisted of nine bands covering the pI range 4.96—5.64. This range is relevant for species identification of heated fish by urea IEF. The kit may also be used for native IEF in the low pH range, as demonstrated by running an extract made from the kit together with water-soluble fish muscle proteins on Servalyt Precotes 3—6. | Descripción: | 6 páginas, 4 tablas, 6 figuras | Versión del editor: | http://dx.doi.org/10.1002/(SICI)1522-2683(20000501)21:8<1458::AID-ELPS1458>3.0.CO;2-T | URI: | http://hdl.handle.net/10261/56989 | DOI: | 10.1002/(SICI)1522-2683(20000501)21:8<1458::AID-ELPS1458>3.0.CO;2-T | ISSN: | 0173-0835 | E-ISSN: | 1522-2683 |
| Aparece en las colecciones: | (IIM) Artículos |
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