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|Title:||Quantitative biomolecular sensing station based on magnetoresistive patterned arrays|
|Authors:||Serrate, David, Teresa, José María de, Marquina, Clara, Marzo, J., Ibarra, M. Ricardo|
|Abstract:||The combination of magnetoresistive sensors and magnetic labeling of bioanalytes, which are selectively captured by their complementary antibody in the proximity of the sensor is a powerful method in order to attain truly quantitative immunological assays. In this paper we present a technical solution to exploit the existing spin valve technology to readout magnetic signals of bio-functionalized magnetic nanoparticles. The method is simple and reliable, and it is based on a discrete scan of lateral flow strips with a precise control of the contact force between sensor and sample. It is shown that the signal of the sensor is proportional to the local magnetization produced by the nanoparticles in a wide range of concentrations, and the sensitivity thresholds in both calibration samples and real immunorecognition assays of human chorionic gonadotropin hormone are well below the visual inspection limit (5.5. ng/ml). Furthermore the sample scanning approach and the reduced dimensions of the sensors provide unprecedented spatial resolution of the nanoparticle distribution across the supporting nitrocellulose strip, therefore enabling on-stick control references and multi-analyte capability. © 2012 Elsevier B.V.|
|Citation:||Biosensors and Bioelectronics 35(1): 206- 212 (2012)|
|Appears in Collections:||(ICMA) Artículos|
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