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Closed Access item Sequencing, characterization and transcriptional analysis of the histidine decarboxylase operon of Lactobacillus buchneri

Authors:Martín, M. Cruz
Fernández García, María
Martínez Linares, Daniel
Álvarez González, Miguel Ángel
Keywords:BA, Biogenic amine, LAB, Lactic Acid Bacteria
Issue Date:2005
Publisher:Society for General Microbiology
Citation:Microbiology 151: 1219-1228 (2005)
Abstract:The amplification of an internal fragment of the hdcA gene for histidine decarboxylase in Lactobacillus buchneri showed the gene to be located on the bacterial chromosome. Reverse PCR was then used to amplify both it and its adjacent genes. The histidine decarboxylase cluster was found to be composed of four genes: hdcC (expressed in Lactococcus lactis, the product of which is located in the membrane, suggesting it to be a histidine/histamine antiporter), hdcA (which encodes histidine decarboxylase), hdcB (of unknown function but co-transcribed as bicistronic mRNA together with hdcA) and hisS (the only copy of a gene encoding a histidyl-tRNA synthetase in Lb. buchneri). The expression of hisS depends on the histidine concentration of the growth medium, and it can be transcribed as monocistronic or hdcA–hdcB–hisS polycistronic mRNA.
Description:The GenBank/EMBL/DDBJ accession number for the sequences reported in this paper is AJ749838.-- Final full-text version of the paper available at: http://dx.doi.org/10.1099/mic.0.27459-0.
Publisher version (URL):http://dx.doi.org/10.1099/mic.0.27459-0
URI:http://hdl.handle.net/10261/5691
ISSN:1350-0872
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Appears in Collections:(IPLA) Artículos

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