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Title

Cytotoxic activity against prelabelled RTG-2 cells in the turbot, Scophthalmus maximus (L.)

AuthorsTafalla, Carolina; Figueras Huerta, Antonio ; Novoa, Beatriz
KeywordsTurbot
Leucocytes
Cytotoxicity
Fish
Non-specific natural cytotoxic cells
Immune system
Issue Date2001
PublisherBlackwell Publishing
CitationJournal of Fish Diseases 24(3): 169-175 (2001)
AbstractLeucocytes derived from head kidney, blood and spleen of turbot, Scophthalmus maximus (L.), exhibited cytotoxic activities against an established cell line (RTG-2) derived from rainbow trout, Oncorhynchus mykiss (Walbaum). The optimal effector:target ratio, temperature and incubation period for the activity was determined. Cytotoxicity was not caused by released factors, as effector:target contact was needed. Visualization of the cultures under the transmission electron microscope corroborated the contact between leucocytes and target cells and suggested the implication of monocyte-like cells in this cell-mediated cytotoxicity.
Description7 páginas, 5 figuras
Publisher version (URL)http://dx.doi.org/10.1046/j.1365-2761.2001.00288.x
URIhttp://hdl.handle.net/10261/56858
DOI10.1046/j.1365-2761.2001.00288.x
ISSN0140-7775
E-ISSN1365-2761
Appears in Collections:(IIM) Artículos
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