Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/5685
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Cytotoxin and Pyrogenic Toxin Superantigen Gene Profiles of Staphylococcus aureus Associated with Subclinical Mastitis in Dairy Cows and Relationships with Macrorestriction Genomic Profiles |
Autor: | Fueyo Mendoza, José María; Mendoza, M. Carmen; Rodicio, M. Rosario; Muñiz, J.; Álvarez González, Miguel Ángel CSIC ORCID ; Martín, M. Cruz CSIC ORCID | Palabras clave: | Staphylococcus aureus Cow's milk Superantigen Genes Gene profiles Subclinical mastitis |
Fecha de publicación: | mar-2005 | Editor: | American Society for Microbiology | Citación: | Journal of Clinical Microbiology 43: 1278-1284 (2005) | Resumen: | A set of 84 Staphylococcus aureus isolates collected from the milk of cows with subclinical mastitis in Asturias (a cattle region of Spain) and six control strains were tested for sequences of genes encoding hemolysins (hla, hlb, hld, hlg, and hlg-2), leukotoxins (lukPV, lukM, and lukED), toxic shock syndrome toxin (tst), and enterotoxins (sea to see, seg to ser, and seu) by conventional and multiplex PCR. It was found that 84, 83, 11, and 39 isolates carried some type of hl, luk, tst, or se gene, respectively, which were arranged in 14 exotoxin genotypes. All of the isolates were negative for lukPV, hlg, sea, sed, see, sej, sek, sep, seq, and ser. Two gene groupings could be related with pathogenicity islands—[lukED, seg, sei, sem, sen, seo ± seu] with Saß-1 and [tst, sec, sel] with SaPIbov, present in 45 and 13.1% of the isolates, respectively—while 11.9% of them carried both islands. Only one contained seb (together with Saß-1), and another contained seh (together with lukED). The isolates were also analyzed by pulsed-field gel electrophoresis performed with SmaI. Thirty-nine SmaI profiles (similarity coefficient [S] = 0.94 to 0.21) were differentiated; 12, 1, and 10 of these, respectively, were generated by isolates presumptively carrying Saß-1, SaPIbov, or both. Five SmaI profiles (S 0.8) formed a cluster, which contained 20 and 10 isolates carrying one (Saß-1) or both islands. These data show the high frequency of genes encoding cytotoxins and pyrogenic toxin superantigens, their relationship with pathogenicity islands, and their distribution among a diversity of genetic types of S. aureus related to subclinical mastitis. | Versión del editor: | http://dx.doi.org/10.1128/JCM.43.3.1278-1284.2005 | URI: | http://hdl.handle.net/10261/5685 | DOI: | 10.1128/JCM.43.3.1278-1284.2005 | ISSN: | 0095-1137 |
Aparece en las colecciones: | (IPLA) Artículos |
Mostrar el registro completo
CORE Recommender
PubMed Central
Citations
20
checked on 19-abr-2024
SCOPUSTM
Citations
75
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
67
checked on 26-feb-2024
Page view(s)
480
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.