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Título : Nisin-controlled expression of Norwalk virus VP60 protein in Lactobacillus casei
Autor : Martín, M. Cruz, Fernández García, María, Martín-Alonso, José M., Parra, Francisco, Boga, José A., Álvarez González, Miguel Ángel
Palabras clave : NICE system
Antigen expression
Norwalk virus
Lactobacillus casei
Fecha de publicación : 15-Aug-2004
Editor: Elsevier
Federation of European Microbiological Societies
Citación : FEMS Microbiology Letters. 237: 385-391 (2004).
Resumen: A food-grade strain with nisRK stably integrated into the genome, was constructed in order to implement the nisin-controlled expression system (NICE) in Lactobacillus casei ATCC393. Expression of β-glucuronidase (gus) reporter gene was employed to optimize the system, which has been successfully used to produce the main antigenic protein from Norwalk virus, opening new perspectives for producing edible vaccines.
Descripción : Final full-text version of the paper available at:
ISSN: 0378-1097
DOI: 10.1016/j.femsle.2004.07.002
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