Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/5676
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Generation of Food-Grade Recombinant Lactic Acid Bacterium Strains by Site-Specific Recombination |
Autor: | Martín, M. Cruz CSIC ORCID ; Alonso, Juan Carlos CSIC ORCID ; Suárez Fernández, Juan Evaristo CSIC; Álvarez González, Miguel Ángel CSIC ORCID | Palabras clave: | Lactic acid bacteria Recombinant microorganism Food industry Plasmids Phage Vector Homologous recombination Genetic engineering Lactobacillus casei Microorganism resistance Fermentation starter Virus Lactobacillaceae Bacteria |
Fecha de publicación: | jun-2000 | Editor: | American Society for Microbiology | Citación: | Applied and Environmental Microbiology 66(6): 2599-2604 (2000) | Resumen: | The construction of a delivery and clearing system for the generation of food-grade recombinant lactic acid bacterium strains, based on the use of an integrase (Int) and a resolvo-invertase (β-recombinase) and their respective target sites (attP-attB and six, respectively) is reported. The delivery system contains a heterologous replication origin and antibiotic resistance markers surrounded by two directly oriented six sites, a multiple cloning site where passenger DNA could be inserted (e.g., the cI gene of bacteriophage A2), the int gene, and the attP site of phage A2. The clearing system provides a plasmid-borne gene encoding β-recombinase. The nonreplicative vector-borne delivery system was transformed into Lactobacillus casei ATCC 393 and, by site-specific recombination, integrated as a single copy in an orientation- and Int-dependent manner into the attB site present in the genome of the host strain. The transfer of the clearing system into this strain, with the subsequent expression of the β-recombinase, led to site-specific DNA resolution of the non-food-grade DNA. These methods were validated by the construction of a stable food-grade L. casei ATCC 393-derived strain completely immune to phage A2 infection during milk fermentation. | URI: | http://hdl.handle.net/10261/5676 | DOI: | 10.1128/AEM.66.6.2599-2604.2000 | ISSN: | 0099-2240 |
Aparece en las colecciones: | (IPLA) Artículos |
Mostrar el registro completo
CORE Recommender
SCOPUSTM
Citations
56
checked on 21-abr-2024
WEB OF SCIENCETM
Citations
55
checked on 27-feb-2024
Page view(s)
344
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.