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Closed Access item Culture-independent approaches for studying viruses from hypersaline environments

Authors:Santos, Fernando
Yarza, Pablo
Parro-García, Víctor
Meseguer, Inmaculada
Rosselló-Mora, Ramón
Antón, Josefa
Issue Date:2012
Publisher:American Society for Microbiology
Citation:Applied and Environmental Microbiology 78: 1635- 1643 (2012)
Abstract:Hypersaline close-to-saturation environments harbor an extremely high concentration of virus-like particles, but the number of haloviruses isolated so far is still very low. Haloviruses can be directly studied from natural samples by using different cultureindependent techniques that include transmission electron microscopy, pulsed-field gel electrophoresis, and different metagenomic approaches. Here, we review the findings of these studies, with a main focus on the metagenomic approaches. The analysis of bulk viral nucleic acids directly retrieved from the environment allows estimations of viral diversity, activity, and dynamics and tentative host assignment. Results point to a diverse and active viral community in constant interplay with its hosts and to a >hypersalineness> quality common to viral assemblages present in hypersaline environments that are thousands of kilometers away from each other. © 2012, American Society for Microbiology.
Publisher version (URL):http://dx.doi.org/10.1128/AEM.07175-11
Identifiers:doi: 10.1128/AEM.07175-11
issn: 0099-2240
e-issn: 1098-5336
Appears in Collections:(IMEDEA) Artículos
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