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Título: | Rapid separation of chlorophylls a and b and their demetallated and dephytylated derivatives using a monolithic silica C18 column and a pyridine-containing mobile phase |
Autor: | Garrido, J. L. CSIC ORCID; Rodríguez Hernández, Francisco José CSIC ORCID; Campaña, Emilia; Zapata, Manuel CSIC | Palabras clave: | Chlorophylls Chlorophyllides Pheophytins Pheophorbides |
Fecha de publicación: | 2003 | Editor: | Elsevier | Citación: | Journal of Chromatography A 994(1-2): 85-92 (2003) | Resumen: | A monolithic C18-bonded silica rod column (Merck Chromolith) was applied to the separation of mixtures of chlorophylls a and b and their derivatives originated by hydrolysis of the phytyl ester linkage (chlorophyllides), loss of the central Mg atom (pheophytins), or both processes (pheophorbides). Mobile phases containing two different ion-pair reagents, ammonium acetate and buffered pyridine, were tested. Both eluents achieved the resolution of the eight pigments in less than 5 min. The method based on the pyridine-containing mobile phase was applied to the separation of chlorophylls and their green coloured degradation products in senescing leaves of deciduous trees, green fruits and a marine microalga. | Descripción: | 8 páginas, 5 figuras | Versión del editor: | http://dx.doi.org/10.1016/S0021-9673(03)00486-2 | URI: | http://hdl.handle.net/10261/56327 | DOI: | 10.1016/S0021-9673(03)00486-2 | ISSN: | 0021-9673 |
Aparece en las colecciones: | (IIM) Artículos |
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